American Journal of Otolaryngology - Head and Neck Medicine and Surgery
Volume 27, Issue 1 , Pages 13-17, January 2006

The effect of donor-specific transfusion upon rejection in a rat model of laryngeal transplantation☆

Head and Neck Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, USA

This study was performed in accordance with the PHS policy on Humane Care and Use of Laboratory Animals, the National Institutes of Health Guide for the Care and Use of Laboratory Animals, and the Animal Welfare Act (7 USC et seq); the animal use protocol was approved by the Institutional Animal Care and Use Committee of the Cleveland Clinic Foundation.

Abstract 

Lifelong immunosuppression carries significant morbidity, and techniques to reduce or eliminate such immunosuppression might expand laryngeal transplantation. This study investigates the ability of donor-specific transfusion to establish tolerance in a rat model of laryngeal transplantation. A total of 289 transplants were performed from Lewis-Brown-Norway donors to Lewis recipients. Donor-specific transfusion was provided as single intravenous injections of donor splenocytes 1 hour before transplantation. Different combinations of in vitro irradiation of donor larynges and postoperative cyclosporine doses provided additional immunomodulation. Allograft rejection was scored histologically at sacrifice 15 or 30 days posttransplant. Multivariate analysis was performed across all groups, and paired analyses compared groups with and without donor-specific transfusion. Donor-specific transfusion did not improve rejection score, although increased cyclosporine dose did (P < .001). Donor splenocyte injection on the day of transplantation does not establish tolerance to laryngeal allografts or permit reduction of other immunosuppression. Other immunomodulatory strategies to establish tolerance in rat laryngeal transplantation require further investigation.

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

☆ This study was supported by a research grant from the Cleveland Clinic Foundation, Cleveland, Ohio.

PII: S0196-0709(05)00109-2

doi:10.1016/j.amjoto.2005.05.013

American Journal of Otolaryngology - Head and Neck Medicine and Surgery
Volume 27, Issue 1 , Pages 13-17, January 2006

Article Tools