Expression of histamine receptors (H1, H2, and H3) in the rabbit endolymphatic sac: an immunohistochemical study

Dagli, Muharrem; Goksu, Nebil; Eryilmaz, Adil; Mocan Kuzey, Gamze; Bayazit, Yildirim; Gun, Banu Dogan; Gocer, Celil

doi:10.1016/j.amjoto.2006.12.003

« Previous Next »American Journal of Otolaryngology - Head and Neck Medicine and Surgery
Volume 29, Issue 1 , Pages 20-23, January 2008

Expression of histamine receptors (H₁, H₂, and H₃) in the rabbit endolymphatic sac: an immunohistochemical study

Muharrem Dagli, MD
- Department of Otolaryngology, Head and Neck Surgery, Ankara Numune Research and Training Hospital, Ankara, Turkey
- Corresponding author. Cemal Gursel Caddesi No. 48/1, Cebeci 06600, Ankara, Turkey. Tel.: +90 312 4307118; fax: +90 312 4358832.
Nebil Goksu, MD
- Department of Otolaryngology, Head and Neck Surgery, Faculty of Medicine, Gazi University, Ankara, Turkey
Adil Eryilmaz, MD
- Department of Otolaryngology, Head and Neck Surgery, Ankara Numune Research and Training Hospital, Ankara, Turkey
Gamze Mocan Kuzey, MD
- Department of Pathology, Faculty of Medicine, Hacettepe University, Ankara, Turkey
Yildirim Bayazit, MD
- Department of Otolaryngology, Head and Neck Surgery, Faculty of Medicine, Gazi University, Ankara, Turkey
Banu Dogan Gun, MD
- Department of Pathology, Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak, Turkey
Celil Gocer
- Department of Otolaryngology, Head and Neck Surgery, Ankara Numune Research and Training Hospital, Ankara, Turkey

Received 15 November 2006

Abstract

Objective

The endolymphatic sac (ES) is part of the membranous labyrinth of the inner ear. Its central role in immunologic activity within the inner ear has been confirmed by numerous studies. The aim of this study was to investigate the expression of histamine receptors (H₁, H₂, H₃) in the rabbit ES.

Methods

A total of 10 healthy male New Zealand white rabbits weighing 2 to 3 kg were used in the experiments. For immunohistochemical studies, immunostaining was performed according to the avidin-biotin-peroxidase complex technique.

Results

Serial sections of the ES of rabbits revealed the presence of H₁, H₂, and H₃ receptor immunoreactivity. Immunoreactive cells for all H₁, H₂, and H₃ were found in the epithelial and subepithelial layers of the duct and the proximal ES. In conclusion, this study showed the immunohistochemical localization of H₁, H₂, and H₃ receptors in the ES of rabbits. These receptors may be important in the homeostasis of the inner ear. In addition, they may be target receptors in the medical treatment of inner ear disorders such as endolymphatic hydrops.