Nuclear localization of mammary serine protease inhibitor (MASPIN): is its impact on the prognosis in laryngeal carcinoma due to a proapoptotic effect?

Marioni, Gino; Giacomelli, Luciano; D'Alessandro, Emiliano; Marchese-Ragona, Rosario; Staffieri, Claudia; Ferraro, Silvia M.; Staffieri, Alberto; Blandamura, Stella

doi:10.1016/j.amjoto.2007.04.010

« Previous Next »American Journal of Otolaryngology - Head and Neck Medicine and Surgery
Volume 29, Issue 3 , Pages 156-162, May 2008

Nuclear localization of mammary serine protease inhibitor (MASPIN): is its impact on the prognosis in laryngeal carcinoma due to a proapoptotic effect?☆

Gino Marioni
- Department of Medical and Surgical Specialties, Section of Otolaryngology, University of Padova, Padova, Italy
- Corresponding author. Department of Medical and Surgical Specialties, Section of Otolaryngology, University of Padova, Via Giustiniani 2, 35100 Padova, Italy. Tel.: +39 49 8218626; fax: +39 49 8752266.
Luciano Giacomelli
- Department of Medical/Diagnostic Sciences and Special Therapies, Section of Anatomic Pathology, University of Padova, Padova, Italy
Emiliano D'Alessandro
- Department of Medical/Diagnostic Sciences and Special Therapies, Section of Anatomic Pathology, University of Padova, Padova, Italy
Rosario Marchese-Ragona
- Department of Medical and Surgical Specialties, Section of Otolaryngology, University of Padova, Padova, Italy
Claudia Staffieri
- Department of Medical and Surgical Specialties, Section of Otolaryngology, University of Padova, Padova, Italy
Silvia M. Ferraro
- Department of Medical and Surgical Specialties, Section of Otolaryngology, University of Padova, Padova, Italy
Alberto Staffieri
- Department of Medical and Surgical Specialties, Section of Otolaryngology, University of Padova, Padova, Italy
Stella Blandamura
- Department of Medical/Diagnostic Sciences and Special Therapies, Section of Anatomic Pathology, University of Padova, Padova, Italy

Received 2 January 2007 published online 17 March 2008.

Abstract

Purpose

Cell death by apoptosis is considered a regulator of cell number; cancer cells are defective in their response to apoptosis. Many potential markers of apoptosis are under study: M30 immunoreactivity is confined to the cytoplasm of apoptotic epithelial cells and is expressed during early apoptosis. Mammary serine protease inhibitor (MASPIN), a suppressor of tumor growth, seems to be involved in the induction of tumour cell apoptosis. The aim of our preliminary study was to investigate, for the first time, the relations between MASPIN subcellular pattern of expression, nuclear MASPIN expression, M30 expression, and prognosis in laryngeal carcinoma.

Materials and methods

Subcellular pattern of distribution of MASPIN and nuclear MASPIN expression were immunohistochemically determined in 66 consecutive cases of laryngeal carcinoma. M30 expression in correspondent carcinoma fields was also calculated.

Results

M30 expression was significantly higher in the group of laryngeal carcinomas with MASPIN nuclear localization (P = .024). Our investigation found a reduced carcinoma recurrence rate in the group of patients with MASPIN nuclear localization (P value = .0086). The log-rank test showed a significantly longer disease-free interval in patients with nuclear MASPIN localization (P = .029).

Conclusions

These preliminary results support the hypothesis of an apoptosis-sensitizing effect of nuclear MASPIN in laryngeal carcinoma with the potential perspective of a clinical use of the tumour suppressive proapoptotic function of MASPIN.