Transforming growth factor β isoforms (TGF-β1, TGF-β2, TGF-β3) messenger RNA expression in laryngeal cancer

Kapral, Malgorzata; Strzalka, Barbara; Kowalczyk, Malgorzata; Jurzak, Magdalena; Mazurek, Urszula; Gierek, Tatiana; Paluch, Jaroslaw; Markowski, Jaroslaw; Swiatkowska, Longina; Weglarz, Ludmila

doi:10.1016/j.amjoto.2007.08.003

« Previous Next »American Journal of Otolaryngology - Head and Neck Medicine and Surgery
Volume 29, Issue 4 , Pages 233-237, July 2008

Transforming growth factor β isoforms (TGF-β1, TGF-β2, TGF-β3) messenger RNA expression in laryngeal cancer☆☆☆

Malgorzata Kapral, PhD
- Department of Biochemistry, Medical University of Silesia, Sosnowiec, Poland
- Corresponding author. Department of Biochemistry, Medical University of Silesia, Narcyzow 1 Street, 41-200 Sosnowiec, Poland. Tel.: +48 32 364 10 27; fax: +48 32 364 10 20.
Barbara Strzalka, PhD
- Department of Molecular Biology and Medical Genetics, Medical University of Silesia, Sosnowiec, Poland
Malgorzata Kowalczyk, MSc
- Department of Molecular Biology and Medical Genetics, Medical University of Silesia, Sosnowiec, Poland
Magdalena Jurzak, PhD
- Department of Biochemistry, Medical University of Silesia, Sosnowiec, Poland
Urszula Mazurek, PhD
- Department of Molecular Biology and Medical Genetics, Medical University of Silesia, Sosnowiec, Poland
Tatiana Gierek, MD, PhD
- Department of Laryngology, Medical University of Silesia, Katowice, Poland
Jaroslaw Paluch, MD, PhD
- Department of Laryngology, Medical University of Silesia, Katowice, Poland
Jaroslaw Markowski, MD, PhD
- Department of Laryngology, Medical University of Silesia, Katowice, Poland
Longina Swiatkowska, MSc
- Department of Instrumental Analyses, Medical University of Silesia, Sosnowiec, Poland
Ludmila Weglarz, PhD
- Department of Biochemistry, Medical University of Silesia, Sosnowiec, Poland

Received 15 March 2007 published online 20 March 2008.

Abstract

Purpose

Cancerogenesis is a multistage process controlled by many cytokines, including growth factors. The aim of the study was the comparison of transcriptional activity of transforming growth factor β (TGF-β) genes in laryngeal squamous cell carcinomas and adjacent nonneoplastic tissues.

Materials and Methods

Tissues samples were obtained from 32 patients with laryngeal squamous cell carcinoma in histologic grades G1 to G3 who underwent surgical treatment at the ENT Clinics of Medical University of Silesia in Katowice, Poland. Quantification of gene expression was performed by real-time quantitative reverse transcriptase polymerase chain reaction technique.

Results

In tumor cells, expression of TGF-β1 and TGF-β2 isoforms (P < .001) was higher than in normal tissues. There was a positive correlation between the expression of TGF-β1 and TGF-β2 genes in tumors (R = 0.78, P = .0000) and adjacent normal tissues (R = 0.77, P = .0000).

Conclusions

The results suggest that TGF-β1 and TGF-β2 messenger RNAs may be useful as molecular markers in distinguishing cancer from nonneoplastic tissues in laryngeal area.