Histopathologic changes in guinea pig mastoid mucosa after topical mitomycin C application followed by mastoidectomy

Ozturk, Levent; Kaygusuz, Irfan; Akpolat, Nusret; Keles, Erol; Karlidag, Turgut; Alpay, Hayrettin Cengiz; Yalcin, Sinasi

doi:10.1016/j.amjoto.2007.09.005

« Previous Next »American Journal of Otolaryngology - Head and Neck Medicine and Surgery
Volume 29, Issue 5 , Pages 300-304, September 2008

Histopathologic changes in guinea pig mastoid mucosa after topical mitomycin C application followed by mastoidectomy

Levent Ozturk, MD
- Department of Otorhinolaryngology, Kahramanmaraş Government Hospital, Kahramanmaraş, Turkey
Irfan Kaygusuz, MD
- Department of Otorhinolaryngology, Firat University Medical Faculty, Elazig, Turkey
Nusret Akpolat, MD
- Department of Pathology, Firat University Medical Faculty, Elazig, Turkey
Erol Keles, MD
- Department of Otorhinolaryngology, Firat University Medical Faculty, Elazig, Turkey
Turgut Karlidag, MD
- Department of Otorhinolaryngology, Firat University Medical Faculty, Elazig, Turkey
Hayrettin Cengiz Alpay, MD
- Department of Otorhinolaryngology, Firat University Medical Faculty, Elazig, Turkey
- Corresponding author. Department of Otorhinolaryngology, Firat University, Medical Faculty, 23200 Elazig, Turkey. Tel.: + 90 424 233 35 55; fax: +90 424 238 80 96.
Sinasi Yalcin, MD
- Department of Otorhinolaryngology, Firat University Medical Faculty, Elazig, Turkey

Received 3 May 2007 published online 16 June 2008.

Abstract

Purpose

This study was performed to investigate the histopathologic changes observed in mastoid cavity of guinea pigs after the application of mitomycin C after mastoidectomy.

Materials and methods

The study was performed on 50 guinea pigs. Unilateral (right ear) mastoidectomy was performed on all guinea pigs. Twenty-five guinea pigs were separated as study group and the remaining were separated as control group. A mitomycin C–soaked sponge was placed in the mastoid cavities of the study group and a dry sponge was placed in those of the control group. Their mastoid cavities were examined histopathologically for absorbable sponge waste, abscess formation, fibrosis, vascularization, polymorphonuclear leukocyte infiltration, edema, lymphoplasmacytic inflammatory infiltration, and granulation tissue.

Results

Absorbable sponge waste, abscess formation, fibrosis, vascularization, edema, and lymphoplasmacytic inflammatory infiltration were not significantly different between the groups. However, polymorphonuclear leukocyte infiltration and granulation tissue were statistically different between the groups.