American Journal of Otolaryngology - Head and Neck Medicine and Surgery
Volume 30, Issue 2 , Pages 95-100 , March 2009

Molecular diagnosis of nasopharyngeal carcinoma using detection of Epstein-Barr virus latent membrane protein-1 gene in cervical metastatic lymph nodes

  • Bijan Khademi, MD

      Affiliations

    • Department of Otolaryngology, Shiraz University of Medical Sciences, Shiraz, Iran
  • ,
  • Jalal Mahmoudi, MD

      Affiliations

    • Department of Otolaryngology, Shiraz University of Medical Sciences, Shiraz, Iran
  • ,
  • Ahmad Monabati, MD

      Affiliations

    • Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran
  • ,
  • Behzad Maghsoudi, MD

      Affiliations

    • Department of Anesthesiology, Shiraz University of Medical Sciences, Shiraz, Iran
  • ,
  • Mohammad J. Ashraf, MD

      Affiliations

    • Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran
  • ,
  • Mohammad Mohammadianpanah, MD

      Affiliations

    • Department of Radiation Oncology, Shiraz University of Medical Sciences, Shiraz, Iran
    • Corresponding Author InformationCorresponding author. Department of Radiation Oncology, Nemazee Hospital, Shiraz 71936-13311, Iran. Tel.: +98 711 6260135; fax: +98 711 6260135.
  • ,
  • Narjes Tabibi, BSc

      Affiliations

    • Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran
  • ,
  • Behnaz Valibeigi, BSc

      Affiliations

    • Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran
  • ,
  • Elham Abedi, BSc

      Affiliations

    • Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran

Received 16 November 2007

References 

  1. Pathmanathan R, Prasad U, Raab-Traub N, et al. Undifferentiated non keratinizing and squamous cell carcinoma of the nasopharynx. Variants of Epstein-Barr-virus–infected neoplasia. Am J Pathol. 1995;146:1355–1367
  2. Cohen JI. Epstein-Barr virus infection. N Engl J Med. 2000;343:481–492
  3. Dickson RI, Flores AD. Nasopharyngeal carcinoma. An evaluation of 134 patients treated between 1971-1980. Laryngoscope. 1985;95:276–283
  4. Pathmanathan R, Prasad U, Raab-Traub N, et al. Clonal proliferation of cells infected with Epstein Barr virus in pre invasive lesions related to nasopharyngeal carcinoma. N Engl J Med. 1995;333:693–698
  5. Goldenberg D, Golz A, Netzer A, et al. Epstein-Barr virus and cancers of the head and neck. Am J Otolaryngol. 2001;22:197–205
  6. Vasef MA, Ferlito A, Weiss LM. Nasopharyngeal carcinoma, with emphasis on its relationship to Epstein-Barr virus. Ann Otol Rhinol Laryngol. 1997;106:348–356
  7. Mendenhall WM, Mancuso AA, Amdur RJ, et al. Squamous cell carcinoma metastatic to the neck from an unknown head and neck primary site. Am J Otolaryngol. 2001;22:261–267
  8. Califano J, Westra WH, Koch W, et al. Unknown primary head and neck squamous cell carcinoma: molecular identification of the site of origin. J Natl Cancer Inst. 1999;91:599–604
  9. Aslani M, Sultanem K, Voung T, et al. Metastatic carcinoma to the cervical nodes from an unknown head and neck primary site: is there a need for neck dissection?. Head Neck. 2007;29:585–590
  10. Chan AT, Teo PM, Johnson PJ. Nasopharyngeal carcinoma. Ann Oncol. 2002;13:1007–1015
  11. Chang YS, Tyan YS, Liu ST, et al. Detection of Epstein-Barr virus DNA amplification. J Clin Microbiol. 1990;28:2398–2402
  12. Hao SP, Tsang NM, Chang KP. Screening nasopharyngeal carcinoma by detection of LMP-1 gene from nasopharyngeal swab. Cancer. 2003;97:1909–1913
  13. Wei WI, Sham JST, Zong YS, et al. The efficacy of fiberoptic endoscopic examination and biopsy in the detection of early nasopharyngeal carcinoma. Cancer. 1991;67:3127–3130
  14. Liavaag PG, Cheung RK, Kerrebijn JD, et al. The physiologic reservoir of Epstein-Barr virus does not map to upper aerodigestive tissues. Laryngoscope. 1998;108:42–46
  15. Baumforth KR, Young LS, Flavell KJ, et al. The Epstein-Barr virus and its association with human cancers. Mol Pathol. 1999;52:307–322
  16. Kobayashi I, Shima K, Saito I, et al. Prevalence of Epstein-Barr virus in oral squamous cell carcinoma. J Pathol. 1999;189:34–39
  17. Hao SP, Tsang NM, Chang KP. Differentiation of recurrent nasopharyngeal carcinoma and skull base osteoradionecrosis by Epstein-Barr virus. Derived latent membrane protein gene. Laryngoscope. 2001;111:650–652
  18. Tsang NM, Chang KP, Lin SY, et al. Detection of Epstein-Barr virus-derived latent membrane protein-1 (LMP-1) gene in various head and neck cancers: is it specific for nasopharyngeal carcinoma?. Laryngoscope. 2003;113:1050–1054
  19. Ren Q, Sato H, Murono S, et al. Epstein-Barr virus (EBV) latent membrane protein 1 induces interleukin-8 through the nuclear factor-kappa B signaling pathway in EBV-infected nasopharyngeal carcinoma cell line. Laryngoscope. 2004;114:855–859
  20. Tugwood JD, Lau WH, O SK, et al. Epstein-Barr virus-specific transcription in normal and malignant nasopharyngeal biopsies and in lymphocytes from healthy donors and infectious mononucleosis patients. J Gen Virol. 1987;68:1081–1091
  21. Hording U, Albeck H, Katholm M, et al. Epstein-Barr virus in exfoliated cells from the postnasal space. Viral detection by polymerase chain reaction is not a useful means of screening for nasopharyngeal carcinoma in high-risk populations. APMIS. 1994;102:367–370
  22. Khabie N, Savva A, Kasperbauer JL, et al. Epstein-Barr virus DNA is not increased in tonsillar carcinoma. Laryngoscope. 2001;111:811–814
  23. Horikawa T, Sheen TS, Takeshita H, et al. Induction of c-Met proto-oncogene by Epstein-Barr virus latent membrane protein-1 and the correlation with cervical lymph node metastasis of nasopharyngeal carcinoma. Am J Pathol. 2001;159:27–33
  24. Baichwal VR, Sugden B. The multiple membrane-spanning segments of the BNLF-1 oncogene from Epstein–Barr virus are required for transformation. Oncogene. 1989;4:67–74
  25. Dolcetti R, Menezes J. Epstein-Barr virus and undifferentiated nasopharyngeal carcinoma: new immunobiological and molecular insights on a long-standing etiopathogenic association. Adv Cancer Res. 2003;87:127–157
  26. Yuge NK, Mochiki M, Nibu K, et al. Detection of Epstein-Barr virus in metastatic lymph nodes of patients with nasopharyngeal carcinoma and a primary unknown cancer. Arch Otolaryngol Head Neck Surg. 2003;129:338
  27. Feinmesser R, Miyazaki I, Chenng R, et al. Diagnosis of nasopharyngeal carcinoma by DNA amplification of tissue obtained by fine-needle aspiration. N Engl J Med. 1992;326:17
  28. Macdonald MR, Freeman JL, Hui MF, et al. Role of Epstein-Barr virus in fine-needle aspirates of metastatic neck nodes in the diagnosis of nasopharyngeal carcinoma. Head Neck. 1995;17:487–493
  29. Goldenberg D, Benoit NE, Begum S, et al. Epstein-Barr virus in head and neck cancer assessed by quantitative polymerase chain reaction. Laryngoscope. 2004;114:1029–1031
  30. Gonzales-Moles MA, Gutierrs J, Rodriguez MJ, et al. Epstein-Barr virus LMP-1 expression in oral squamous cell carcinoma. Laryngoscope. 2002;112:482–487
  31. Shimakage M, Horii K, Tempaku A, et al. Association of Epstein-Barr virus with oral cancers. Hum Pathol. 2002;33:608–614
  32. Maeda T, Hiranuma H, Matsumura S, et al. Epstein-Barr virus infection and response to radiotherapy in squamous cell carcinoma of the oral cavity. Cancer Lett. 1998;125:25–30
  33. D'Costa J, Saranath D, Sanghi V, et al. Epstein-Barr virus in tobacco-induced oral cancers and oral lesion in patients from India. J Oral Pathol Med. 1998;27:78–82
  34. Curz I, Van den Brule AJ, Steenbergen RD, et al. Prevalence of Epstein-Barr virus in oral squamous cell carcinomas, premalignant lesions and normal mucosa—a study using the polymerase chain reaction. Oral Oncol. 1997;33:182–188
  35. Mizugaki Y, Sugawara Y, Shinozaki F, et al. Detection of Epstein-Barr virus in oral papilloma. Jpn J Cancer Res. 1998;89:604–607
  36. van Heerden WE, van Rensburg EJ, Engelbrecht S, et al. Prevalence of EBV in oral squamous cell carcinomas in young patients. Anticancer Res. 1995;15:2335–2339
  37. Mao EJ, Smith CJ. Detection of Epstein-Barr virus DNA by the polymerase chain reaction (PCR). J Oral Pathol Med. 1993;22:12–17

 Conflicts of interest: The authors disclose any commercial or other associations that might pose a conflict of interest in connection with submitted manuscript.

PII: S0196-0709(08)00042-2

doi: 10.1016/j.amjoto.2008.02.013

American Journal of Otolaryngology - Head and Neck Medicine and Surgery
Volume 30, Issue 2 , Pages 95-100 , March 2009