Biofilm formation by Pseudomonas aeruginosa on ossicular reconstruction prostheses☆
Presented at the American Academy of Otolaryngology-Head and Neck Surgery Annual Meeting, September 16–19, 2007, Washington, DC.
Received 22 May 2008 published online 09 March 2009.
Abstract
Purpose
Ossicular chain reconstruction may be complicated by prosthesis extrusion. As prostheses are commonly placed in middle ears contaminated with biofilm-forming bacteria, such as Pseudomonas aeruginosa (PA), extrusion may be caused by development of a biofilm on the prosthesis and the host response to this biofilm. The purpose of this experiment was to determine if PA forms biofilm on different ossicular chain reconstruction prostheses to a different degree.
Methods
Prostheses made of titanium, hydroxylapatite (HA), and plastic (23 each) were cultured with PA in broth for 96 hours. Biofilm formation was assessed by electron microscopy and quantitative microbiology.
Results
Titanium prostheses formed less biofilm than plastic (P = .0003) and HA (P = .003), but there was no difference between HA and plastic. Correction for surface area did not alter these significant differences.
Conclusions
Pseudomonas aeruginosa forms biofilm on ossicular prostheses, particularly those made of plastic and HA. These differences could, in part, explain the extrusion propensity of certain biomaterials.
Department of Otolaryngology-Head and Neck Surgery, University of Florida College of Medicine, Gainesville, FL, USA
Corresponding author. Department of Otolaryngology, University of Florida, Box 100264, 1600 SW Archer Rd, Gainesville, FL 32610-0264, USA. Tel.: +1 352 392 4461; fax: +1 352 392 6781.
☆ Eric M. Jaryszak, MD, PhD, Stryker (Kalamazoo, MI) 2006 and 2007 Resident travel grant awardee, Medtronic ENT (Jacksonville, FL) grant support. Patrick J. Antonelli, MD, Grace Medical (Memphis, TN)-patent royalties, Sharklet Technologies LLC (Alachua, FL)-grant support, Medtronic ENT-grant support, paid consultant.
ABSTRACT