American Journal of Otolaryngology - Head and Neck Medicine and Surgery
Volume 32, Issue 1 , Pages 32-37 , January 2011

Biofilms in chronic suppurative otitis media and cholesteatoma: scanning electron microscopy findings

  • James Saunders, MD

      Affiliations

    • Section of Otolaryngology, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA
    • Corresponding Author InformationCorresponding author. Section of Otolaryngology, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA. Tel.: +1 603 650 8124; fax: +1 603 650 0052.
    • ,
  • Michael Murray, MD

      Affiliations

    • Camino Ear, Nose and Throat Clinic, San Jose, CA, USA
    • ,
  • Anthony Alleman, MD

      Affiliations

    • Department of Radiology, University of Oklahoma Health Science Center, Oklahoma City, OK, USA

Received 10 September 2009

References 

  1. Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of persistent infections. Science. 1999;284:1318–1322
  2. Costerton JW, Geesey GG, Cheng KJ. How bacteria stick. Sci Am. 1978;238:86–95
  3. Whittaker CJ, Klier CM, Kolenbrander PE. Mechanisms of adhesion by oral bacteria. Annu Rev Microbiol. 1996;50:513–552
  4. Costerton JW, Lewandowski Z, Caldwell DE, et al. Microbial biofilms. Annu Rev Microbiol. 1995;49:711–745
  5. Post JC, Hiller NL, Nistico L, et al. The role of biofilms in otolaryngologic infections: update 2007. Curr Opin Otolaryngol Head Neck Surg. 2007;15:347–351
  6. Vlastarakos PV, Nikolopoulos TP, Maragoudakis P, et al. Biofilms in ear, nose and throat infections: how important are they?. Laryngoscope. 2007;117:668–673
  7. Sanderson AR, Leid JG, Hunsaker D. Bacterial biofilms on the sinus mucosa of human subjects with chronic rhinosinusitis. Laryngoscope. 2006;116:1121–1126
  8. Ramadan HH. Chronic rhinosinusitis and bacterial biofilms. Curr Opin Otolaryngol Head Neck Surg. 2006;14:183–186
  9. Post JC. Direct evidence of bacterial biofilms in otitis media. Laryngoscope. 2001;111:2083–2094
  10. Ehrlich GD, Veeh R, Wang X, et al. Mucosal biofilm formation in middle-ear mucosa in the chinchilla model of otitis media. JAMA. 2002;287:1710–1715
  11. Fergie N, Bayston R, Pearson JP, et al. Is otitis media with effusion a biofilm infection?. Clin Otolaryngol Allied Sci. 2004;29:38–46
  12. Hall-Stoodley L, Hu FZ, Gieseke A, et al. Direct detection of bacterial biofilms on the middle-ear mucosa of children with chronic otitis media. JAMA. 2006;296:202–211
  13. Dohar JE, Hebda PA, Veeh R, et al. Mucosal biofilm formation on middle-ear mucosa in a nonhuman primate model of chronic suppurative otitis media. Laryngoscope. 2005;115:1469–1472
  14. Chole RA, Faddis BT. Evidence for microbial biofilms in cholesteatomas. Arch Otolaryngol Head Neck Surg. 2002;128:1129–1133
  15. Barakate M, Beckenham E, Curotta J, et al. Bacterial biofilm adherence to middle-ear ventilation tubes: scanning electron micrograph images and literature review. J Laryngol Otol. 2007;121:993–997
  16. Palmer RJ, Sternberg C. Modern microscopy in biofilm research: confocal microscopy and other approaches. Curr Opin Biotechnol. 1999;10:263–268
  17. Lawrence JR, Neu TR. Confocal laser scanning microscopy for analysis of microbial biofilms. Methods Enzymol. 1999;310:131–144
  18. Paterson-Beedle M, Nott KP, Macaskie LE, et al. Study of biofilm within a packed-bed reactor by three-dimensional magnetic resonance imaging. Methods Enzymol. 2001;337:285–305
  19. Tater EC, Unal FO, Tatar I, et al. Investigation of surface changes in different types of ventilation tubes using scanning electron microscopy and correlation of findings with clinical follow-up. Int J Pediatr Otorhinolaryngol. 2006;70:411–417
  20. Wang EW, Jung JY, Pashia ME, et al. Otopathogenic Pseudomonas aeruginosa strains as competent biofilm formers. Arch Otolaryngol Head Neck Surg. 2005;131:983–989
  21. Lenz AP, Williamson KS, Pitts B, et al. Localized gene expression in Pseudomonas aeruginosa biofilms. Appl Environ Microbiol. 2008;74:4463–4471

☆ Presented at the Middle Section of the Triological Society 2005.

☆☆ Funded by a Resident Research Grant from the Triological society.

★ The authors have no conflicting interests in the publication of this paper, and funding was internal within the Otolaryngology Department of The University of Oklahoma Health Science Center.

PII: S0196-0709(09)00216-6

doi: 10.1016/j.amjoto.2009.09.010

American Journal of Otolaryngology - Head and Neck Medicine and Surgery
Volume 32, Issue 1 , Pages 32-37 , January 2011

Article Tools

* Image Usage & Resolution