Similar cellular proliferation activities in nasal polyps and adjacentinferior turbinate

Wu, Chia-Chen; Lee, Ta-Jen; Chang, Po-Hung; Tsai, Chi-Neu; Lee, Yun-Shien; Fu, Chia-Hsiang; Huang, Chi-Che

doi:10.1016/j.amjoto.2010.12.001

« Previous Next »American Journal of Otolaryngology - Head and Neck Medicine and Surgery
Volume 33, Issue 1 , Pages 14-19, January 2012

Similar cellular proliferation activities in nasal polyps and adjacentinferior turbinate

Chia-Chen Wu, MD
- Department of Otolaryngology – Head and Neck Surgery, Chang Gung Memorial Hospital, Kwei-Shan, Tao-Yuan, Taiwan
Ta-Jen Lee, MD
- Department of Otolaryngology – Head and Neck Surgery, Chang Gung Memorial Hospital, Kwei-Shan, Tao-Yuan, Taiwan
Po-Hung Chang, MD
- Department of Otolaryngology – Head and Neck Surgery, Chang Gung Memorial Hospital, Kwei-Shan, Tao-Yuan, Taiwan
- Graduate Institute of Clinical Medical Sciences, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan
Chi-Neu Tsai, PhD
- Graduate Institute of Clinical Medical Sciences, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan
Yun-Shien Lee, PhD
- Genomic Medicine Research Core Laboratory, Chang Gung Memorial Hospital, Kwei-Shan, Tao-Yuan, Taiwan
- Department of Biotechnology, Ming-Chuan University, Kwei-Shan, Tao-Yuan, Taiwan
Chia-Hsiang Fu, MD
- Department of Otolaryngology – Head and Neck Surgery, Chang Gung Memorial Hospital, Kwei-Shan, Tao-Yuan, Taiwan
Chi-Che Huang, MD
- Department of Otolaryngology – Head and Neck Surgery, Chang Gung Memorial Hospital, Kwei-Shan, Tao-Yuan, Taiwan
- Department of Otolaryngology-Head and Neck Surgery, Chang Gung Memorial Hospital, Keelung, Chang Gung University, Taoyuan, Taiwan
- Corresponding author. Department of Otolaryngology – Head and Neck Surgery, Chang Gung Memorial Hospital, Kwei-Shan, Tao-Yuan, Taiwan No 5, Fu Hsing Street, Kwei-Shan, Taoyuan 333, Taiwan. Tel.: +886 3 3281200x8466; fax: +886 3 3271244.

Received 28 June 2010 published online 07 March 2011.

Abstract

Introduction

Nasal polyps (NP) are regulated by proinflammatory transcription factors such as activator protein-1 (AP-1), which comprises members of the proto-oncogene Jun and Fos protein families. The binding of AP-1 proteins to the 12-O-tetradecanoylphorbol-13-acetate (TPA)-response element can activate target genes and regulate many critical cellular processes. The proliferating cell nuclear antigen (PCNA) gene contains AP-1 sites, and its expression is regulated by AP-1 activity. In this study, NP and inferior turbinate (IT) were evaluated, compared with normal mucosa, to see if diffuse inflammation and active cellular proliferation exist.

Materials and Methods

A diseased group of 20 subjects and control group of 20 subjects were enrolled in this study. NP and IT were evaluated with expression of phospho-c-Jun, c-Fos, PCNA, major basic protein by immunohistochemistry, and eosinophil numbers by cell counts.

Result

The expression of phospho-c-Jun, c-Fos, PCNA, major basic protein, and eosinophil numbers showed no significant difference in IT and NP of the same patients, but all were significantly higher in IT and NP compared with normal mucosa (P < .05).

Conclusion

Our result demonstrated strong evidence that diffuse mucosal inflammation and active cellular proliferation do exist in rhinosinusitis with nasal polyposis. As the degree of the disease severity increases, the difference of eosinophilic infiltration and cellular proliferation activity between NP and its adjacent mucosa decreases. An integrated anti-inflammatory treatment may be more important than surgical intervention.